LSBio
Polyclonal Rabbit anti-Human TJP1 / ZO-1 Antibody (WB)

Western blot analysis of extracts of various cell lines, using TJP1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 15s.

Polyclonal Rabbit anti-Human TJP1 / ZO-1 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human TJP1 / ZO-1 antibodies →
$281.00each
Catalog Number: LS-C746966-20

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Overview

Description
ZO-1 antibody LS-C746966 is an unconjugated rabbit polyclonal antibody to ZO-1 (TJP1) from human. It is reactive with human and mouse. Validated for WB.
Application
WB
Reactivity
Hu, Ms
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ZO-1 | Zona occludens protein 1 | Zona occludens 1 | Zonula occludens protein 1 | Tight junction protein 1 | ZO1 | Tight junction protein ZO-1 | TJP1 | Zonula occludens 1 protein
Recommended Dilution: WB
1:200 - 1:500
UniProt Number
Q07157
SwissProt
Q07157

Target

Target
Human TJP1 / ZO-1
Antigen Species
Hu
Gene Name
TJP1

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1600-1700 of human TJP1 (NP_003248.3). PKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMC
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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