LSBio
Monoclonal Mouse anti-Human TIMP4 Antibody (clone 9:4-7, IHC, WB)

Immunohistochemistry of Mouse anti-TMIP4 antibody. Tissue: kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TMIP4 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TMIP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Monoclonal Mouse anti-Human TIMP4 Antibody (clone 9:4-7, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse
Format:Liquid
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$311.00each
Catalog Number: LS-C744845-25

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Overview

Description
TIMP4 antibody LS-C744845 is an unconjugated mouse monoclonal antibody to TIMP4 from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu, Ms
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Metalloproteinase inhibitor 4 | TIMP-4 | TIMP4
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
5 - 10 µg/ml
Recommended Dilution: WB
1:2000 - 1:10000
UniProt Number
Q99727
SwissProt
Q99727

Target

Target
Human TIMP4
Antigen Species
Hu
Epitope
This antibody reacts with human TIMP4 in tissue sections of formalin-fixed, paraffin-embedded In ELISAs and Native-PAGE, this antibody does not cross-react with recombinant human TIMP-1, -2 or -3. A BLAST analysis was used to suggest cross-reactivity with TIMP4 from human and mouse sources based on the immunizing sequence. Cross-reactivity with TIMP-4 from other sources has not been determined.
Gene Name
TIMP4

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
9:4-7
Isotype
IgG2b
Light Chain
κ
Origin Species
Hu

Immunogen

Immunogen
Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full length human TIMP4 protein followed by hybridoma development.
Immunogen Type
Recom

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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