LSBio
Polyclonal Goat anti-E. coli Sucrose Phosphorylase Antibody (Liquid, WB)

Goat anti Sucrose Phosphorylase antibody was used to detect purified Sucrose Phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System

Polyclonal Goat anti-E. coli Sucrose Phosphorylase Antibody (Liquid, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity:E. coli
Format:Liquid
See all E. coli Sucrose Phosphorylase antibodies →
$311.00each
Catalog Number: LS-C744689-25

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Overview

Description
Sucrose Phosphorylase antibody LS-C744689 is an unconjugated goat polyclonal antibody to e. coli Sucrose Phosphorylase. Validated for ELISA, IP and WB.
Application
ELISA, IP, WB
Reactivity
E.col
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:5000 - 1:20000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:5000

Target

Target
E. coli Sucrose Phosphorylase
Antigen Species
E.col
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.coli]. Cross reactivity against Sucrose Phosphorylase from other sources is unknown.

Antibody

Host Species
Gt
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Sucrose Phosphorylase [E.coli]

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
IEX

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

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