Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
OPN is cleaved by MMP to yield 2 fragments, which migrate at 40 kDa (N terminal) and 32 kDa (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs. The epitope that is recognized by Rabbit-anti-Osteopontin is shown in violet. This antibody detects the full length OPN and the 32 kDa fragment. It does not recognize the 40 kDa fragment.
Polyclonal Rabbit anti-Human SPP1 / Osteopontin Antibody (IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Dog, Human, Mouse, Pig, Rat |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744596-25
Catalog Number: LS-C744596
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Osteopontin antibody LS-C744596 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Can, Hu, Ms, Por, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BSPI | BNSP | Bone sialoprotein 1 | ETA-1 | Nephropontin | OPN | Secreted phosphoprotein 1 | SPP-1 | SPP1/CALPHA1 fusion | Urinary stone protein | Uropontin | Osteopontin | Bone sialoprotein I | SPP1
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IHC
- 1:100 - 1:300
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P10451
- NCBI GenBank
- NM_000582, NP_000573.1
- SwissProt
- P10451
Target
- Target
- Human SPP1 / Osteopontin
- Antigen Species
- Hu
- Epitope
- The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
- Gene Name
- SPP1
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Origin Species
- Hu
Immunogen
- Immunogen
- This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
- Concentration
- 75 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Crude
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
