Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
The SMAD pathway follows the canonical TGF- signaling pathway. TGF- dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD (R-SMAD).i.e. SMAD2 or SMAD3. The R-SMAD then binds to the common SMAD (coSMAD) i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.
Polyclonal Rabbit anti-Human SMAD3 Antibody (phospho-Thr179, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745346-25
Catalog Number: LS-C745346
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- SMAD3 antibody LS-C745346 is an unconjugated rabbit polyclonal antibody to SMAD3 (pThr179) from human. It is reactive with human and mouse. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | SMAD3 | HSMAD3 | Mad protein homolog | SMAD 3
- Recommended Dilution: ELISA
- 1:15000 - 1:75000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P84022
- NCBI GenBank
- NM_005902, NP_005893.1
- SwissProt
- P84022
Target
- Target
- Human SMAD3
- Antigen Species
- Hu
- Epitope
- Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
- Gene Name
- SMAD3
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-SMAD3 pT179 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
