LSBio
Polyclonal Goat anti-Human SERPINA1 / Alpha 1 Antitrypsin Antibody (Lyophilized, Biotin, WB)

Western Blot of goat anti-Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 µg per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4°C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: Blocking Buffer for Fluorescent Western Blotting - MB-070 overnight at 4°C.

Polyclonal Goat anti-Human SERPINA1 / Alpha 1 Antitrypsin Antibody (Lyophilized, Biotin, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Lyophilized
See all Human SERPINA1 / Alpha 1 Antitrypsin antibodies →
$564.00each
Catalog Number: LS-C744752-100

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Overview

Description
Alpha 1 Antitrypsin antibody LS-C744752 is a biotin-conjugated goat polyclonal antibody to human Alpha 1 Antitrypsin (SERPINA1). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
A1A | A1AT | AAT | Alpha 1 Antitrypsin | Alpha-1 protease inhibitor | Alpha-1-antiproteinase | Alpha-1-antitrypsin | Alpha1AT | PRO2275 | Alpha-1-antitrypsin null | SERPINA1 | PI1 | Serpin A1 | Alpha 1 AT
Recommended Dilution: ELISA
1:50000
Recommended Dilution: WB
1:2000 - 1:10000
UniProt Number
P01009
NCBI GenBank
AAB59495.1, K02212
SwissProt
P01009

Target

Target
Human SERPINA1 / Alpha 1 Antitrypsin
Antigen Species
Hu
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified a-1-anti-Trypsin [Human Plasma]. Cross reactivity against a-1-anti-Trypsin from other sources is unknown.
Gene Name
SERPINA1

Antibody

Host Species
Gt
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
A1-Anti-Trypsin [Human Plasma].

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
IEX

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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