Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Purified anti-CD162 (Clone KPL-1) overnight at 4°C. On the next day, tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG antibody (Clone POLY4053, red) for an hour at room temperature. Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph software.