Human SELE / CD62E / E-selectin

Rat

Human (tested or 100% immunogen sequence identity)

Monoclonal

4D10

FITC

Affinity purified

Unmodified

LPS-stimulated human endothelial cells

The antigen is CD62E, the epitope has not been characterized. Antigen distribution: Isolated cells: Positive on LPS, TNFa, IL-1, or TPA stimulated endothelial cells (HUVECs) in tissue culture. No cross reactivity with other cell types known. Tissue sections: Strongly positive on human endothelial cells in acutely inflamed tissues such as atopic dermatitis or contact dermatitis 24h after sensitization; in the healthy spleen and lymph node. Few single cells express the 4D10 antigen. The antigen is generally absent in chronically inflamed tissues. Biochemistry: The antigen is a 589 amino acid transmembrane protein. It consists of a C-type lectin-like domain, an epidermal growth factor (EGF)-like domain, six short consensus repeat (SCR) domains, a transmembrane domain and a cytoplasmic tail. CD62E is a counter-receptor for sialyl Lewis X, sialyl Lewis a and CLA (cutaneous lymphocyte antigen). It is involved in adhesion between myeloid cells and activated endothelium, and supports leucocytes rolling. Some skin-associated memory T cells express CLA, and it may be that CD62E is also involved in their homing to cutaneous sites of chronic inflammation. In addition, CD62E may also be involved in tumour metastasis. A soluble form of CD62E has been reported to mediate angiogenesis in pathological disorders. Specificity: Human: CD62E. Other: Not tested.

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS pH 7.2, 10mg/ml BSA, 0.1% sodium azide

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16581 NCBI: NM_000450 NP_000441.2