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Catalog Number Size Price
LS-C817141-100 100 µl $412 
RNMT / HCM Antibody - Immunofluorescence staining of RNMT in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RNMT polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
RNMT / HCM Antibody - Anti-RNMT rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: HEK-293 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 57 kDa.
RNMT / HCM Antibody - RNMT was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-RNMT rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RNMT rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 60 kDa.
RNMT / HCM Antibody - Immunofluorescence staining of RNMT in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RNMT polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
RNMT / HCM Antibody - Anti-RNMT rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: HEK-293 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 57 kDa.
RNMT / HCM Antibody - RNMT was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-RNMT rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RNMT rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 60 kDa.
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Polyclonal Rabbit anti‑Human RNMT / HCM Antibody (IF, WB) LS‑C817141

Polyclonal Rabbit anti‑Human RNMT / HCM Antibody (IF, WB) LS‑C817141

Antibody:
RNMT / HCM Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$412
LS-C817141-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RNMT / HCM Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HCM antibody LS-C817141 is an unconjugated rabbit polyclonal antibody to human HCM (RNMT). Validated for ICC, IF, IP and WB.
Target
Human RNMT / HCM
Synonyms
RNMT | Hcm1p | HCMT1c | HMet | HCM | HCMT1 | MRNA cap methyltransferase | KIAA0398 | RG7MT1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human RNMT fragment
Specificity
Human RNMT
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RNMT / HCM
O43148 NM_003799 NP_003790.1

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Requested From: United States
Date Requested: 5/18/2024