Western Blot of Mouse Anti-RFP antibody. Lane 1: YFP protein. Lane 2: Prestained Molecular Weight Marker. Lane 3: Reduced RFP control Protein. Lane 4: Reduced mCherry. Lane 5: GFP protein. Lane 6: Non-Reduced RFP control Protein. Lane 7: Non-Reduced mCherry. Load: 300ng per lane. Primary antibody: RFP antibody at 1:2000 in MB-070 for 3 hours at RT. Secondary antibody: HRP anti-Mouse secondary antibody at 1:10,000 in MB-070 for 60 min at RT. Substrate: TMBM-100 for 20 min. Predicted/Observed size: ~27 kDa.
RFP / Red Fluorescent Protein Antibody (clone 8E5.G7)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744819-25
Catalog Number: LS-C744819
For bulk orders or custom formulations:
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- Description
- Red Fluorescent Protein antibody LS-C744819 is an unconjugated mouse monoclonal antibody to Red Fluorescent Protein (RFP). Validated for ELISA, Flow, IF, IP and WB.
- Application
- ELISA, FC, IF, IP, WB
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:75000 - 1:150000
- Recommended Dilution: WB
- 1:1000 - 1:10000
Target
- Target
- RFP / Red Fluorescent Protein
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 8E5.G7
- Isotype
- IgG2a
- Light Chain
- κ
Immunogen
- Immunogen
- Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
- Immunogen Type
- Fusion-P
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl