Western blot of 20× UV treated HeLa lysate showing specific immunolabeling of the ~65 kDa NF-?B p65 phosphorylated at Ser316 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).
RELA / NFKB p65 Antibody (phospho-Ser316)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$479.00each
Catalog Number: LS-C772954-100
Catalog Number: LS-C772954
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- Description
- NFKB p65 antibody LS-C772954 is an unconjugated rabbit polyclonal antibody to NFKB p65 (RELA) (pSer316) from human. It is reactive with human and mouse. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Bov, Can, Gt, Ham, Por, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q04206
- NCBI GenBank
- NM_021975, NP_068810.3
- SwissProt
- Q04206
Target
- Target
- Human RELA / NFKB p65
- Antigen Species
- Hu
- Gene Name
- RELA
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Origin Species
- Hu
Immunogen
- Immunogen
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser316 conjugated to KLH
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 10 mM HEPES pH7.5, 150 mM NaCl, 50% Glycerol
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year.
- Recommended Storage Buffer
- 10 mM HEPES|150 mM NaCl