LSBio
Rabbit Anti-Mouse IgG2b Secondary Antibody (AP)

Dot Blot of Rabbit anti-Mouse IgG2b Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG2b. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Mouse IgG2b Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT.

Rabbit Anti-Mouse IgG2b Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Mouse
Format:Liquid
See all Mouse IgG2b antibodies →
$426.00each
Catalog Number: LS-C60798-1

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Overview

Description
Mouse IgG2b antibody LS-C60798 is an AP-conjugated rabbit polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Ms
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:8000 - 1:32000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:1000 - 1:3000

Target

Target
Mouse IgG2b
Target Heavy Chain
IgG2b
Antigen Species
Ms
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Mouse IgG2b heavy chain.

Format

Conjugate/Label
AP
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.09% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.