LSBio
Rabbit Anti-Human IgG Fc Secondary Antibody (AP)

Dot Blot of Human IgG Fc Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG Fc. Load: Lane 1 - 100ng. Lane 2 - 33.33ng. Lane 3 - 11.11ng. Lane 4 - 3.70ng. Lane 5 - 1.23ng. Primary antibody: n/a. Secondary antibody: Human IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.

Rabbit Anti-Human IgG Fc Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Human
Format:Liquid
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$400.00each
Catalog Number: LS-C60611-1

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Overview

Description
Human IgG Fc antibody LS-C60611 is an AP-conjugated rabbit polyclonal antibody. Validated for DB, ELISA, IE, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2500

Target

Target
Human IgG Fc
Target Heavy Chain
IgG
Antigen Species
Hu
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit.
Immunogen Type
Native

Format

Conjugate/Label
AP
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.05% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.