LSBio
Rabbit Anti-Horse IgG Secondary Antibody (HRP)

ELISA results of purified Rabbit anti-Horse IgG Antibody Peroxidase Conjugated tested against purified Horse IgG. Each well was coated in duplicate with 1.0 µg of Horse IgG The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate

Rabbit Anti-Horse IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Horse
Format:Lyophilized
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$360.00each
Catalog Number: LS-C60480-2

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Overview

Description
Horse IgG antibody LS-C60480 is an HRP-conjugated rabbit polyclonal antibody. Validated for ELISA, IE, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Hr
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:50000 - 1:200000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:5000 - 1:20000

Target

Target
Horse IgG
Target Heavy Chain
IgG
Antigen Species
Hr
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG and Horse Serum.

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Horse IgG whole molecule was produced by repeated immunization with Horse IgG whole molecule in rabbit.
Immunogen Type
Native

Format

Conjugate/Label
HRP
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.