LSBio
Rabbit Anti-Horse IgG Secondary Antibody (AP)

ELISA results of purified Goat anti-Human IgG antibody Alk Phos Conjugation. Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using NPP-B500 buffer, Streptavidin HRP conjugate at 1:10,000 and NPP-10 substrate.

Rabbit Anti-Horse IgG Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Horse
Format:Liquid
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$376.00each
Catalog Number: LS-C60483-1

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Overview

Description
Horse IgG antibody LS-C60483 is an AP-conjugated rabbit polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Hr
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:2000 - 1:20000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2500

Target

Target
Horse IgG
Target Heavy Chain
IgG
Antigen Species
Hr
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG and Horse Serum.

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Horse IgG whole molecule
Immunogen Type
Native

Format

Conjugate/Label
AP
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.1% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.