LSBio
Polyclonal Rabbit anti-Bovine PRSS1 / Trypsin Antibody (Liquid, Biotin, IHC, WB)

Western Blot of rabbit anti-Trypsin Antibody Biotin Conjugated. Lane 1: Trypsin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Trypsin Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 26 kDa, 26 kDa for Trypsin. Other band(s): autocatalytic fragment of Trypsin, ~10 kDa.

Polyclonal Rabbit anti-Bovine PRSS1 / Trypsin Antibody (Liquid, Biotin, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Bovine
Format:Liquid
See all Bovine PRSS1 / Trypsin antibodies →
$311.00each
Catalog Number: LS-C745049-25

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Overview

Description
Trypsin antibody LS-C745049 is a biotin-conjugated rabbit polyclonal antibody to bovine Trypsin (PRSS1). Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Bov
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Beta-trypsin | Cationic trypsinogen | Digestive zymogen | HPC | Hereditary pancreatitis | TRP1 | TRY4 | Trypsin | Trypsinogen i | TRYP1 | Trypsinogen | TRY1 | Trypsinogen 1 | Nonfunctional trypsin 1 | Protease serine 1 | PRSS1 | Serine protease 1 | Trypsin I | Trypsin-1 | Trypsinogen A
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2500
UniProt Number
P07477
SwissProt
P07477

Target

Target
Bovine PRSS1 / Trypsin
Antigen Species
Bov
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas]. Cross reactivity against Trypsin from other sources may occur but has not been specifically determined.
Gene Name
PRSS1

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
Immunogen Type
Native

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
IEX

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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