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Polyclonal Rabbit anti-Human PRKCB / PKC-Beta Antibody (WB)

Western blot using the affinity purified anti-PKC beta antibody shows detection of PKC beta in ~25 µg of U251 whole cell lysate (glioma derived). A 4-12% gel was used for separation. The arrowhead corresponds to 76 kDa PKC beta. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BSA in TTBS at 4° C, overnight.

Polyclonal Rabbit anti-Human PRKCB / PKC-Beta Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human PRKCB / PKC-Beta antibodies →
$311.00each
Catalog Number: LS-C745316-25

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Overview

Description
PKC-Beta antibody LS-C745316 is an unconjugated rabbit polyclonal antibody to PKC-Beta (PRKCB) from human. It is reactive with human and monkey. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Predicted Reactivity
Bov, NHP, Rb
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
PKC beta 2 | Pkc betaII | PRKCB2 | Protein kinase c beta | Protein kinase c beta 2 | Protein kinase c beta i | Protein kinase C beta type | Protein kinase C, beta | Protein kinase C, beta 1 | PRKCB1 | Pkc betaI | PKC-beta | PKCB | Pkcbeta | PkcbetaI | PRKCB | PKC-B | PkcbetaII | Protein kinase c beta 1 | Protein kinase c beta II
Recommended Dilution: ELISA
1:75000 - 1:80000
Recommended Dilution: WB
1:1000 - 1:2000
UniProt Number
P05771
NCBI GenBank
NM_002738, NP_002729.2
SwissProt
P05771

Target

Target
Human PRKCB / PKC-Beta
Antigen Species
Hu
Epitope
This antibody is specific for human PKC beta protein. A BLAST analysis was used to suggest cross-reactivity with PKC beta from human and monkey sources based on a 100% homology with the immunizing sequence. Expect partial cross reactivity to PKC beta from rabbit and bovine (93% homology) and rat and mouse (87% homology) sources. Cross-reactivity with PKC beta from other sources has not been determined.
Gene Name
PRKCB

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.