Fluorescent image of HeLa cell stained with PRKAA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). PRKAA2 immunoreactivity is localized to Cytoplasm significantly.

Monoclonal Mouse anti-Human PRKAA2 / AMPK Alpha 2 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Immunofluorescence, Western Blot
Reactivity:	Human
Format:	Liquid

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$401.00each

Catalog Number: LS-C162144-400

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: AMPK Alpha 2 antibody LS-C162144 is an unconjugated mouse monoclonal antibody to human AMPK Alpha 2 (PRKAA2). Validated for IF and WB.
Application: IF, WB
Reactivity: Hu
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: ACACA kinase | Alpha2 AMPK | AMPK alpha 2 | AMPK subunit alpha-2 | Acetyl-CoA carboxylase kinase | AMPK-2 | AMPKa2 | AMPKalpha2 | Ampk alpha2 | AMPK2 | PRKAA | AMPK | AMPK-alpha-2 chain | PRKAA2 | HMGCR kinase
Recommended Dilution: IF/ICC: 1:10 - 1:50
Recommended Dilution: WB: 1:100 - 1:500
UniProt Number: P54646
NCBI GenBank: NM_006252, NP_006243.2
SwissProt: P54646

Target

Target: Human PRKAA2 / AMPK Alpha 2
Antigen Species: Hu
Epitope: This PRKAA2 monoclonal antibody is generated from mouse immunized with PRKAA2 recombinant protein.
Gene Name: PRKAA2

Antibody

Host Species: Ms
Clonality: Monoclonal
Isotype: IgG2a
Light Chain: κ

Format

Conjugate/Label: Unconj
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Volume: 400 Microliter
Preservative: Yes
Purification Method: Prot G

Storage & Handling

Storage Conditions: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer: PBS