Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
PLK1 / PLK-1 Antibody (clone OTI6C6, Carrier-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Dog, Human |
| Format: | Lyophilized |
$522.00each
Catalog Number: LS-C784228-100
Catalog Number: LS-C784228
For bulk orders or custom formulations:
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- Description
- PLK-1 antibody LS-C784228 is an unconjugated mouse monoclonal antibody to PLK-1 (PLK1) from human. It is reactive with human and dog. Validated for IHC, IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Can, Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- PLK1 | Polo like kinase | Polo-like kinase (Drosophila) | Polo-like kinase 1 | STPK13 | Polo (Drosophia)-like kinase | PLK | PLK-1
- Recommended Dilution: IHC
- 1:150
- Recommended Dilution: IP
- 4 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P53350
- NCBI GenBank
- NM_005030, NP_005021.2
- SwissProt
- P53350
Target
- Target
- Human PLK1 / PLK-1
- Antigen Species
- Hu
- Gene Name
- PLK1
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- OTI6C6
- Isotype
- IgG2b
- Origin Species
- Hu
Immunogen
- Immunogen
- Full length human recombinant protein of human PLK1 (NP_005021) produced in HEK293T cell.
- Immunogen Type
- FL-RP
Format
- Conjugate/Label
- Unconj
- Format
- Lyophilized
- Formulation
- PBS, pH 7.3, 8% Trehalose
- Preservative
- No
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS