Western Blot of rabbit Anti-CD31 Antibody. Lane 1: 3T3 Whole cell lysate. Lane 2: Jurkat Whole cell lysate. Lane 3: HT-29 Whole cell lysate. Load: 10µg/lane. Primary Antibody Anti-CD-31 used 1:500 with 0.75% TBS with Casein overnight. Secondary Antibody Goat anti-rabbit HRP used 1:40,000 for 30 min at room temp. Expect MW: 80kda.
PECAM-1 / CD31 Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Rat |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744612-25
Catalog Number: LS-C744612
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- Description
- CD31 antibody LS-C744612 is an unconjugated rabbit polyclonal antibody to CD31 (PECAM-1) (C-Terminus) from rat. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Rt
- Predicted Reactivity
- Hu, Ms
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM1 | PECAM-1 | GPIIA
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: IHC
- 4 µg/ml
- Recommended Dilution: WB
- 1:500
- UniProt Number
- P16284
- NCBI GenBank
- NM_000442, NP_000433.4
- SwissProt
- P16284
Target
- Target
- Rat PECAM-1 / CD31
- Antigen Species
- Rt
- Epitope
- A BLAST analysis was used to suggest cross-reactivity with CD31 from rat, human, and mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Gene Name
- PECAM1
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 90 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Crude
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl