Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 Antibodies. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load 5 ? per lane. Primary Antibody: Blot A: Anti-PARP1- n term; Blot B: Anti-PARP1- internal at 1?/mL for overnight at 4?. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4?. Predicted/Observed size: 113 kDa for endogenous PARP1. Other band(s): nonspecific ~ 40kDa in PARP1-AD only.
PARP1 Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Other, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745018-100
Catalog Number: LS-C745018
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- PARP1 antibody LS-C745018 is an unconjugated rabbit polyclonal antibody to human PARP1 (C-Terminus). Validated for IE and WB.
- Application
- Other, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ADPRT | ADP-ribosyltransferase NAD(+) | Adp-ribosyltransferase | ADPRT1 | ARTD1 | ADPRT 1 | Poly(ADP-ribose) synthetase | Poly(ADP-ribosyl)transferase | PARP | Poly [ADP-ribose] polymerase 1 | PADPRT-1 | PARP-1 | PARP1 | Poly (ADP-ribose) polymerase 1 | Poly(ADP-ribose) polymerase | Poly[ADP-ribose] synthase 1 | PPOL
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P09874
- NCBI GenBank
- NM_001618, NP_001609.2
- SwissProt
- P09874
Target
- Target
- Human PARP1
- Antigen Species
- Hu
- Epitope
- This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined.
- Gene Name
- PARP1
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- PARP1 (internal) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with c-terminus region of human PARP1 autocatalytic domain recombinant protein.
- Immunogen Type
- Recom
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
