LSBio
Polyclonal Rabbit anti-Human NOTCH2 Antibody (N-Terminus, IHC, WB)

Anti-NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Polyclonal Rabbit anti-Human NOTCH2 Antibody (N-Terminus, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human NOTCH2 antibodies →
$311.00each
Catalog Number: LS-C744599-25

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Overview

Description
NOTCH2 antibody LS-C744599 is an unconjugated rabbit polyclonal antibody to human NOTCH2 (N-Terminus). Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AGS2 | HN2 | Notch (Drosophila) homolog 2 | Notch homolog 2 (Drosophila) | Notch 2 | Notch homolog 2 | NOTCH2 | HJCYS
Recommended Dilution: ELISA
1:30000 - 1:90000
Recommended Dilution: IHC
1:500
Recommended Dilution: WB
1:400 - 1:2000
UniProt Number
Q04721
NCBI GenBank
NM_024408, NP_077719.2
SwissProt
Q04721

Target

Target
Human NOTCH2
Antigen Species
Hu
Epitope
This antiserum is directed against human NOTCH 2. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2. Only one amino acid difference (conservative) occurs in mouse and rat.
Gene Name
NOTCH2

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 2 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
70 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Crude

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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