Target
Human NGFR / CD271 / TNR16
Synonyms
NGFR | CD271 | CD271 antigen | gp80-LNGFR | Nerve growth factor receptor | p75 ICD | p75(NTR) | p75NTR | TNFR superfamily, member 16 | NGF receptor | TNFRSF16 | p75-NTR
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
FITC.
Also available
Unconjugated or conjugated with
Biotin.
Purification
Protein A purified
Immunogen
Recombinant soluble human CD271.
Specificity
Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.
Applications
- Flow Cytometry
- ELISA
- Applications tested for the base form of this product only
Usage
Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 ul of 300 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of product at a 1:50 dilution (10 ug/ml). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.55 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Storage
Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NGFR / CD271 / TNR16