Human NGFR / CD271 / TNR16

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

ANC271/3D7

Biotin. Also available Unconjugated or conjugated with FITC.

Protein A purified

Unmodified

Recombinant soluble human CD271.

Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.

• Flow Cytometry
• ELISA
• Applications tested for the base form of this product only

Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 ul of 300 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD271/Biotin at a concentration of 10 ug/ml. Cells were then washed twice and incubated with secondary reagent Streptaviden/R-PE. They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08138 NCBI: NM_002507 NP_002498.1