All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
NFKB1 / NF-Kappa-B Antibody (N-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744633-25
Catalog Number: LS-C744633
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- NF-Kappa-B antibody LS-C744633 is an unconjugated rabbit polyclonal antibody to human NF-Kappa-B (NFKB1) (N-Terminus). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Ebp- 1 | EBP-1 | NF-KappaB p50 | NFkappaB | NFKB-p50 | KBF1 | NF-kappa-B | NF-kappaB | NF-kB1 | NFKB-p105 | p105 | p50 | DNA binding factor KBF1 | DNA-binding factor KBF1 | NF-kappabeta | NFKB1
- Recommended Dilution: ELISA
- 1:5000 - 1:25000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P19838
- NCBI GenBank
- NM_003998, NP_003989.2
- SwissProt
- P19838
Target
- Target
- Human NFKB1 / NF-Kappa-B
- Antigen Species
- Hu
- Gene Name
- NFKB1
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- Antiserum, 0.01% Sodium Azide
- Concentration
- 80 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.