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Antibodies > NEFM / NF-M > LS-C204562
 
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Catalog Number Size Price
LS-C204562-100 100 µl (21.5 mg/ml) $376 
NEFM / NF-M Antibody - View of mixed neuron/glial cultures stained with NEFM / NF-M antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
NEFM / NF-M Antibody - Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NEFM / NF-M antibody, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.
NEFM / NF-M Antibody - View of mixed neuron/glial cultures stained with NEFM / NF-M antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
NEFM / NF-M Antibody - Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NEFM / NF-M antibody, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.
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Polyclonal Chicken anti‑Human NEFM / NF‑M Antibody (IF, WB) LS‑C204562

Polyclonal Chicken anti‑Human NEFM / NF‑M Antibody (IF, WB) LS‑C204562

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Antibody:
NEFM / NF-M Chicken anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204562-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NEFM / NF-M Chicken anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NF-M antibody LS-C204562 is an unconjugated chicken polyclonal antibody to human NF-M (NEFM). Validated for ICC, IF and WB.
Target
Human NEFM / NF-M
Synonyms
NEFM | 160 kDa neurofilament protein | NEF3 | NF-M | NFM | Neurofilament 3
Host
Chicken
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgY Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • ICC (1:2000)
  • Immunofluorescence (1:2000)
  • Western blot (1:10000)
Usage
The IgY solution can be used at dilutions of at least 1:2000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.
Presentation
PBS, 10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEFM / NF-M
P07197 NM_005382 NP_005373.2

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NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PathPlus™ NEFM / NF‑M Antibody (clone RNF403) LS‑B7183
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Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PathPlus™ NEFM / NF‑M Antibody (clone RNF406) LS‑B7184
Species: Human, Monkey, Guinea pig, Hamster, Rabbit, Xenopus
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
NEFM / NF-M Antibody - IHC of paraffin-embedded Human embryonic brain cortex tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF‑M Antibody (aa413‑773, clone OTI5B7) LS‑C339771
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
NEFM / NF-M Antibody - Immunohistochemistry analysis of the neurofilament medium chain showing staining in the filaments of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament medium chain monoclonal antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
NEFM / NF‑M Antibody (clone RMO 14.9) LS‑C354909
Species: Rat, Human, Mouse, Rabbit, Chicken
Applications: IHC, Immunofluorescence, Western blot, ELISA
See all NEFM / NF-M Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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