Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti-NEDD1 in Western Blot using the Anti-NEDD1 Antibody shows detection of a 73 kDa band corresponding to endogenous NEDD1 in lysates of S phase HeLa cells silenced for either control Luciferase or NEDD1. In right lane (NEDD1i): lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-NEDD1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection.
NEDD1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744651-25
Catalog Number: LS-C744651
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- NEDD1 antibody LS-C744651 is an unconjugated rabbit polyclonal antibody to human NEDD1. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bird, Bov, Ck, Cyno, Fish, Marm, Ms, NHP, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GCP-WD | NEDD-1 | NEDD1 | TUBGCP7 | Protein NEDD1
- Recommended Dilution: ELISA
- 1:100000
- Recommended Dilution: WB
- 1:10000
- UniProt Number
- Q8NHV4
- SwissProt
- Q8NHV4
Target
- Target
- Human NEDD1
- Antigen Species
- Hu
- Epitope
- This antibody reacts with endogenous Nedd1 protein. A BLAST analysis was used to suggest reactivity with Nedd1 from human, chimpanzee, macaque, marmoset, cattle, rat, and mouse based on a 100% homology with the immunizing sequence. Expect partial reactivity with Nedd1 from turkey, chicken, salmon, and Danio based on a 91% homology with the immunizing sequence. Cross-reactivity with Nedd1 from other sources has not been determined.
- Gene Name
- NEDD1
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.
- Immunogen Type
- Recom
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 40 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
