LSBio
Polyclonal Rabbit anti-Human NCOA3 / SRC-3 / AIB1 Antibody (IHC, WB)

Immunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.

Polyclonal Rabbit anti-Human NCOA3 / SRC-3 / AIB1 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$311.00each
Catalog Number: LS-C745310-25

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Overview

Description
AIB1 antibody LS-C745310 is an unconjugated rabbit polyclonal antibody to human AIB1 (NCOA3 / SRC-3). Validated for ELISA, IHC, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Cynomolgus Monkey, Dog, Mouse, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ACTR | AIB1 | BHLHe42 | CAGH16 | CBP-interacting protein | CTG26 | AIB-1 | P/CIP | TNRC14 | TRAM-1 | NCOA3 | Nuclear receptor coactivator 3 | PCIP | RAC-3 | SRC3 | TNRC16 | KAT13B | NCoA-3 | SRC-3
Recommended Dilution: ELISA
1:175000
Recommended Dilution: IHC
5 µg/ml
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
Q9Y6Q9
SwissProt
Q9Y6Q9

Target

Target
Human NCOA3 / SRC-3 / AIB1
Antigen Species
Human
Epitope
This antibody reacts with several isoforms of human NCOA3 protein (a, b, d, e and f). A BLAST analysis was used to suggest cross-reactivity with NCOA3 from human and macaque sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with NCOA3 from dog, bovine, mouse and rat sources based on partial sequence homology. Cross-reactivity with NCOA3 from other sources has not been determined.
Gene Name
NCOA3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.82 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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