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Catalog Number Size Price
LS-C662249-10 10 µg $318 
LS-C662249-100 100 µg $470 
NCAN / Neurocan Antibody - Immunohistochemistry - Anti-Neurocan Picoband Antibody
NCAN / Neurocan Antibody - Immunohistochemistry - Anti-Neurocan Picoband Antibody
NCAN / Neurocan Antibody - Western blot analysis of Neurocan using anti-Neurocan antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurocan antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Neurocan at approximately 200KD. The expected band size for Neurocan is at 143KD.
NCAN / Neurocan Antibody - Western blot - Anti-Neurocan Picoband Antibody
NCAN / Neurocan Antibody - Immunohistochemistry - Anti-Neurocan Picoband Antibody
NCAN / Neurocan Antibody - Immunohistochemistry - Anti-Neurocan Picoband Antibody
NCAN / Neurocan Antibody - Western blot analysis of Neurocan using anti-Neurocan antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurocan antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Neurocan at approximately 200KD. The expected band size for Neurocan is at 143KD.
NCAN / Neurocan Antibody - Western blot - Anti-Neurocan Picoband Antibody
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Polyclonal Rabbit anti‑Human NCAN / Neurocan Antibody (IHC, WB) LS‑C662249

Polyclonal Rabbit anti‑Human NCAN / Neurocan Antibody (IHC, WB) LS‑C662249

Antibody:
NCAN / Neurocan Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662249-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NCAN / Neurocan Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Neurocan antibody LS-C662249 is an unconjugated rabbit polyclonal antibody to human Neurocan (NCAN). Validated for IHC and WB.
Target
Human NCAN / Neurocan
Synonyms
NCAN | Neurocan | Neurocan core protein | Neurocan proteoglycan | NEUR | CSPG3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence of human Neurocan (HENTWIGLNDRIVERDFQWTDNTGLQFENWRENQPDNFFA).
Specificity
Brain.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NCAN / Neurocan
O14594 NM_004386 NP_004377.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024