Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of rabbit Anti-Myosin pS19/pS20 antibody. Lane 1: HeLa whole cell lysate. Lane 2: HeLa whole cell lysate + smooth muscle recombinant phospho protein. Lane 3: HeLa whole cell lysate + regulatory light chain recombinant phospho protein. Load: 10 ug of whole cell lysate + 1.0 ug of recombinant protein. Primary antibody: Mysoin pS19/pS20 antibody at 1.0 ug/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for RLC.
Myosin Antibody (phospho-Ser19/20)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C744598-100
Catalog Number: LS-C744598
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Myosin antibody LS-C744598 is an unconjugated rabbit polyclonal antibody to human Myosin (pSer19/20). Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IHC
- 2.5 µg/ml
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:1000 - 1:5000
Target
- Target
- Human Myosin
- Antigen Species
- Hu
- Epitope
- This antiserum is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 70 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Crude
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
