Western Blot of rabbit anti-RONpY1360 antibody. Lane 1: Phospho RON rProtein (cytoplasmic domain). Lane 2: Phospho RON rProtein (cytoplasmic domain) incubated with RONpY1360 peptide. Load: 0.05 µg per lane. Primary antibody: RON pY1360 antibody at 1µg for overnight at 4°C. Secondary antibody: HRP Goat anti-rabbit IgG secondary antibody at 1:40,000 for 45 min at RT. Block: MB-070 Fluorescent blocking buffer overnight at 4°C. Predicted/Observed size: 152 kDa (full legnth)/~75 kDa (cytoplasmic domain) RON pY1360. Other band(s): none.
MST1R / RON Antibody (phospho-Tyr1360)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745359-25
Catalog Number: LS-C745359
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- Description
- RON antibody LS-C745359 is an unconjugated rabbit polyclonal antibody to human RON (MST1R) (pTyr1360). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-met-related tyrosine kinase | CD136 | CDw136 | CD136 antigen | Friend virus susceptibility 2 | Fv2 | MSP receptor | p185-Ron | PTK8 protein tyrosine kinase 8 | RON | Soluble RON variant 3 | Soluble RON variant 1 | MST1R | MST1R variant RON30 | Oncogene RON | PTK8 | MST1R variant RON62 | Protein-tyrosine kinase 8 | RON variant E2E3 | Soluble RON variant 2 | Soluble RON variant 4
- UniProt Number
- Q04912
- NCBI GenBank
- NM_002447 | NP_002438.2
- SwissProt
- Q04912
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:2000 - 1:10000 |
| WB | 1:500 - 1:2000 |
Target
- Target
- Human MST1R / RON
- Antigen Species
- Human
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for human RON protein phosphorylated at Y1360. Cross-reactivity against RON from other species may occur but has not yet been tested.
- Gene Name
- MST1R
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-RON pY1360 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1360 of human RON protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.79 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl