Western Blot of rabbit Anti-ERK1 N-Term Antibody. Lane 1: Molecular weight marker. Lane 2: ERK1 double mutant WC Lysate. Lane 3: ERK2 protein. Lane 4: Mouse Brain WC Lysate. Lane 5: HEK293 WC Lysate. Load: 10ng lysate, 50ng protein. Blocking: Universal Blocking Buffer (MB-073) for 30 min RT. Primary antibody: Anti-ERK1 N-Term at 1µg/mL in MB-073overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP 1:40,000 in MB-073 for 30 min at RT. Expect: ~43kDa.
MAPK3 / ERK1 Antibody (N-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745383-100
Catalog Number: LS-C745383
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- Description
- ERK1 antibody LS-C745383 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
- Application
- ELISA, WB
- Predicted Reactivity
- Hu, Ms, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | MAPK3 | PRKM3
- Recommended Dilution: ELISA
- 5 ug/ml
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- P27361
- NCBI GenBank
- CAA42744.1, X60188
- SwissProt
- P27361
Target
- Target
- Human MAPK3 / ERK1
- Antigen Species
- Hu
- Epitope
- Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.
- Gene Name
- MAPK3
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-Terminus conjugated to KLH.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl