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Polyclonal Rabbit anti-Human MAPK3 / ERK1 Antibody (N-Terminus, WB)

Western Blot of rabbit Anti-ERK1 N-Term Antibody. Lane 1: Molecular weight marker. Lane 2: ERK1 double mutant WC Lysate. Lane 3: ERK2 protein. Lane 4: Mouse Brain WC Lysate. Lane 5: HEK293 WC Lysate. Load: 10ng lysate, 50ng protein. Blocking: Universal Blocking Buffer (MB-073) for 30 min RT. Primary antibody: Anti-ERK1 N-Term at 1µg/mL in MB-073overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP 1:40,000 in MB-073 for 30 min at RT. Expect: ~43kDa.

Polyclonal Rabbit anti-Human MAPK3 / ERK1 Antibody (N-Terminus, WB)

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Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Format:Liquid
See all Human MAPK3 / ERK1 antibodies →
$311.00each
Catalog Number: LS-C745382-25

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Overview

Description
ERK1 antibody LS-C745382 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Application
ELISA, WB
Predicted Reactivity
Hu, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | MAPK3 | PRKM3
Recommended Dilution: ELISA
1:40000
Recommended Dilution: WB
1 µg/ml
UniProt Number
P27361
NCBI GenBank
CAA42744.1, X60188
SwissProt
P27361

Target

Target
Human MAPK3 / ERK1
Antigen Species
Hu
Epitope
Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.
Gene Name
MAPK3

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-Terminus conjugated to KLH.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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