Western blot of Erk 1,2 activation in untreated PC12 cells (A); cells treated with EGF-100 ng/ml, 5 min (B); PMA-100 nM, 30 min (C); in serum free DMEM, and EGF-100 ng/ml, 5min with 10% FBS in DMEM (D). Wells were equally loaded with 50 ug of who
MAPK1 / ERK2 Antibody (phospho-Thr180/Tyr182)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$368.00each
Catalog Number: LS-C83672-50
Catalog Number: LS-C83672
For bulk orders or custom formulations:
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- Description
- ERK2 antibody LS-C83672 is an unconjugated rabbit polyclonal antibody to ERK2 (MAPK1) (pThr180/Tyr182) from human. It is reactive with human, mouse and rat. Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | MAPK1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
- UniProt Number
- P28482
- NCBI GenBank
- NM_002745 | NP_002736.3
- SwissProt
- P28482
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:20000 - 1:100000 |
Target
- Target
- Human MAPK1 / ERK2
- Antigen Species
- Human
- Gene Name
- MAPK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Peptide derived from the protein area including conserved pT-E-pY motif of activated Erk 1,2.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 20 mM Tris-HCl, pH 8.0, 10 mg/ml BSA, 0.05% sodium azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
- Recommended Storage Buffer
- 20 mm tris-hcl