Western Blot of Anti-MEK1 Antibody. Lane 1: Molecular Weight Marker. Lane 2: MEK1 recombinant lysate. Lane 3: MEK2 recombinant lysate. Load: 10µg. Primary Antibody: Anti-MEK1 (c-term) at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit peroxidase conjugated antibody at 1:40,000 for 30 minutes RT. Blocking: BlockOut Universal Blocking buffer MB-073. Predicted Size: 43.5kDa.
MAP2K1 / MKK1 / MEK1 Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745387-100
Catalog Number: LS-C745387
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- Description
- MEK1 antibody LS-C745387 is an unconjugated rabbit polyclonal antibody to MEK1 (MAP2K1 / MKK1) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Predicted Reactivity
- Hu, Ms, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERK activator kinase 1 | MAP2K1 | MAPK/ERK kinase 1 | MAPKK 1 | MKK1 | PRKMK1 | MEK 1 | MAP kinase kinase 1 | MAPKK1 | MEK1
- Recommended Dilution: ELISA
- 1:40000
- Recommended Dilution: IHC
- 1:100
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- Q02750
- NCBI GenBank
- NM_002755, NP_002746.1
- SwissProt
- Q02750
Target
- Target
- Human MAP2K1 / MKK1 / MEK1
- Antigen Species
- Hu
- Epitope
- Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
- Gene Name
- MAP2K1
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-Terminus conjugated to KLH.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl