Western Blot of rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.
LYZ / Lysozyme Antibody (Liquid, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Chicken |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745111-25
Catalog Number: LS-C745111
For bulk orders or custom formulations:
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- Description
- Lysozyme antibody LS-C745111 is an HRP-conjugated rabbit polyclonal antibody to chicken Lysozyme (LYZ). Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Ck
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 1,4-beta-N-acetylmuramidase C | LZM | Lysozyme | Lysozyme (renal amyloidosis) | LYZ | Renal amyloidosis | Lysozyme C | Gal d 4 | P00698
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: IHC
- 1:500 - 1:2500
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P61626
- NCBI GenBank
- NM_000239, NP_000230.1
- SwissProt
- P61626
Target
- Target
- Chicken LYZ / Lysozyme
- Antigen Species
- Ck
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White]. Cross reactivity against Lysozyme from other tissues and species may occur but have not been specifically determined.
- Gene Name
- LYZ
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-Lysozyme Antibody was produced by repeated immunizations with hen egg white Lysozyme protein.
Format
- Conjugate/Label
- HRP
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- IEX
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl