Immunoprecipitation(IP) of LDHA by using monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-LDHA antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
LDHA / LDH1 Antibody (clone OTI2D11, Carrier-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$522.00each
Catalog Number: LS-C784372-100
Catalog Number: LS-C784372
For bulk orders or custom formulations:
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- Description
- LDH1 antibody LS-C784372 is an unconjugated mouse monoclonal antibody to human LDH1 (LDHA). Validated for IHC, IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- LDH muscle subunit | LDH1 | LDHA | Lactate dehydrogenase M | LDH-M | GSD11 | Lactate dehydrogenase A | LDH-A | LDHM | PIG19 | Proliferation-inducing gene 19
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: IP
- 4 µg/ml
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P00338
- NCBI GenBank
- NM_005566, NP_005557.1
- SwissProt
- P00338
Target
- Target
- Human LDHA / LDH1
- Antigen Species
- Hu
- Gene Name
- LDHA
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- OTI2D11
- Isotype
- IgG1
- Origin Species
- Hu
Immunogen
- Immunogen
- Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.
- Immunogen Type
- FL-RP
Format
- Conjugate/Label
- Unconj
- Format
- Lyophilized
- Formulation
- PBS, pH 7.3, 8% Trehalose
- Preservative
- No
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS