Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, Biotin)
Catalog Number: LS-C810634
2 sizes / from $243.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry, Immunofluorescence
Reactivity: Mouse
Format: Liquid
IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody. Cytokeratin 19 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Cytokeratin 19 antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Monoclonal Mouse anti-Human KRT19 / CK19 / Cytokeratin 19 Antibody (IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756650-100
Catalog Number: LS-C756650
For bulk orders or custom formulations:
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- Description
- Cytokeratin 19 antibody LS-C756650 is an unconjugated mouse monoclonal antibody to human Cytokeratin 19 (KRT19 / CK19). Validated for IF, IHC and WB.
- Application
- IF, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CK19 | Cytokeratin 19 | Cytokeratin-19 | Keratin 19 | KRT19 | Keratin-19 | CK-19 | K19 | K1CS | Keratin, type I, 40-kd
- Recommended Dilution: IF/ICC
- 2 µg/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 0.1 - 0.5 µg/ml
- UniProt Number
- P08727
- NCBI GenBank
- NM_002276, NP_002267.2
- SwissProt
- P08727
Target
- Target
- Human KRT19 / CK19 / Cytokeratin 19
- Antigen Species
- Hu
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- KRT19
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Isotype
- IgG1
- Origin Species
- Hu
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-Terminus of human Cytokeratin 19 (334-372 aa QLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSR), different from the related mouse and rat sequences by nine amino acids.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl
