LSBio
Polyclonal Rabbit anti-Human IPAF / NLRC4 Antibody (aa838-874, WB)

CARD12 antibody Western blot. All lanes: Anti CARD12 at 0.5 ug/ml. Lane 1: 22RV1 Whole Cell Lysate at 40 ug. Lane 2: RH35 Whole Cell Lysate at 40 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 116 kD. Observed band size: 116 kD.

Polyclonal Rabbit anti-Human IPAF / NLRC4 Antibody (aa838-874, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human, Mouse, Rat
Format:Lyophilized
See all Human IPAF / NLRC4 antibodies →
$480.00each
Catalog Number: LS-C407885-100

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Overview

Description
NLRC4 antibody LS-C407885 is an unconjugated rabbit polyclonal antibody to NLRC4 (IPAF) (aa838-874) from human. It is reactive with human, mouse and rat. Validated for WB. Cited in 1 publication.
Application
WB
Reactivity
Hu, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CARD12 | CLANA | CLANB | CLANC | CLAND | CLAN | CLR2.1 | Clan protein | CLAN1 | Ice protease-activating factor | NOD-like receptor C4 | NLRC4 | ICE-protease activating factor | IPAF
UniProt Number
Q9NPP4
SwissProt
Q9NPP4

Target

Target
Human IPAF / NLRC4
Antigen Species
Hu
Epitope
Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
Gene Name
NLRC4

Antibody

Host Species
Rb
Clonality
Polyclonal

Immunogen

Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human CARD12 (838-874 aa KILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDR), different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Recommended Storage Buffer
NaCl
Suppression of Inflammasome Activation by IRF8 and IRF4 in cDCs Is Critical for T Cell Priming. Margaret M McDaniel, Leah C Kottyan, Harinder Singh, Chandrashekhar Pasare. Cell reports. 2020 May;31:107604. PubMed: 32375053 PMC: PMC7325595

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