Western blot using protein-A purified anti-Cat IL-8 antibody shows detection of recombinant Cat IL-8 at 8.9 kDa, raised in yeast. Primary antibody was diluted to 1 µg/ml. 3% BSA from BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody (Goat anti-Rabbit IgG IRDye® 800) was used at 1:20,000.
IL8 / Interleukin 8 Antibody (Liquid)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Cat |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745212-25
Catalog Number: LS-C745212
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- Description
- Interleukin 8 antibody LS-C745212 is an unconjugated rabbit polyclonal antibody to cat Interleukin 8 (IL8). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Fel
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 3-10C | AMCF-I | B-ENAP | C-X-C motif chemokine 8 | GCP-1 | IL-8 | IL8 | Interleukin-8 | K60 | LECT | GCP1 | LUCT | NAF | NAP1 | Neutrophil-activating factor | Interleukin 8 | Protein 3-10C | MDNCF | TSG-1 | CXCL8 | Emoctakin | LYNAP | MONAP | NAP-1 | SCYB8 | T-cell chemotactic factor
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P10145
- NCBI GenBank
- NM_000584 | NP_000575.1
- SwissProt
- P10145
Target
- Target
- Cat IL8 / Interleukin 8
- Antigen Species
- Cat
- Epitope
- This antibody is specific for cat IL-8 protein. A BLAST analysis was used to suggest cross-reactivity with IL-8 from cat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-8 from other sources has not been determined.
- Gene Name
- CXCL8
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 79 amino acids of mature cat IL-8 protein.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.13 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl