Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
IHC analysis of IL36 alpha using anti-IL36 alpha antibody. IL36 alpha was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-IL36 alpha Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IL36A Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756606-100
Catalog Number: LS-C756606
For bulk orders or custom formulations:
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- Description
- IL36A antibody LS-C756606 is an unconjugated rabbit polyclonal antibody to IL36A from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- FIL1 | FIL1 epsilon | IL-1F6 | IL1(EPSILON) | IL1E | IL1F6 | Interleukin 1, epsilon | Interleukin 36, alpha | Interleukin-1 epsilon | Interleukin-1 family member 6 | FIL1(EPSILON) | FIL1E | IL-1 epsilon | IL-1F6 (FIL-1-epsilon) | IL36A | Interleukin-36 alpha
- Recommended Dilution: ELISA
- 1 - 5 ug/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 0.1 - 0.5 µg/ml
- UniProt Number
- Q9UHA7
- NCBI GenBank
- NM_014440 | NP_055255.1
- SwissProt
- Q9UHA7
Target
- Target
- Human IL36A
- Antigen Species
- Human
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- IL36A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived human IL36 alpha recombinant protein (Position: K6-D151).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl
