Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Immunohistochemistry with Rabbit anti-Mouse IL-18 antibody showing IL-18 staining in inflammatory cells of the mucous corium of mouse colon at 20x and 40x. Slide A is a negative control. Slides B and C show staining. Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with mouse anti-IL-18 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 4 universal AP polymer detection system and visualized with WARP RED.
Polyclonal Rabbit anti-Mouse IL18 Antibody (IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Neutralization, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745265-25
Catalog Number: LS-C745265
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- Description
- IL18 antibody LS-C745265 is an unconjugated rabbit polyclonal antibody to mouse IL18. Validated for ELISA, IF, IHC, Neut and WB.
- Application
- ELISA, IF, IHC, Neut, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- IGIF | IL-1g | Interleukin-18 | IFN-gamma-inducing factor | IL-1 gamma | IL-18 | IL18 | Iboctadekin | IL1F4 | Interleukin-1 gamma
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: IF/ICC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q14116
- NCBI GenBank
- NM_001562 | NP_001553.1
- SwissProt
- Q14116
Target
- Target
- Mouse IL18
- Antigen Species
- Mouse
- Epitope
- This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays. This antibody will also recognize the 24,000 inactive precursor form of mouse IL-18. In general, this antibody also detects rat IL-18 in the same formats using similar dilutions. A control of similarly diluted LOW ENDOTOXIN CONTROL RABBIT IgG (code # 011-001-297) is recommended.
- Gene Name
- IL18
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
