LSBio
Polyclonal Rabbit anti-Human IL-10 Antibody (WB)

Western Blot of rabbit anti-IL-10 antibody. Lane 1: Recombinant Human IL-10. Load: 50 ng per lane. Primary antibody: IL-10 antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for IL-10.

Polyclonal Rabbit anti-Human IL-10 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Western Blot
Reactivity:Human
Format:Liquid
See all Human IL-10 antibodies →
$311.00each
Catalog Number: LS-C744647-25

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Overview

Description
IL-10 antibody LS-C744647 is an unconjugated rabbit polyclonal antibody to human IL-10. Validated for ELISA, Flow and WB.
Application
ELISA, FC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CSIF | GVHDS | IL-10 | IL10 | IL10A | Interleukin-10 | Interleukin 10 | TGIF
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: WB
1:2000 - 1:10000
UniProt Number
P22301
SwissProt
P22301

Target

Target
Human IL-10
Antigen Species
Human
Epitope
The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10.
Gene Name
IL10

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
The whole rabbit serum was prepared by repeated immunizations with human IL-10.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.01% Sodium Azide
Concentration
80 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

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