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Catalog Number Size Price
LS-B1347-0.05 0.05 mg (1 mg/ml) $545 
p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human small intestine. Positive staining observed within the absorptive epithelium bordering the villi. Less staining observed within the lamina propria.   Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human spleen. Positive staining observed within a subset of lymphocytes.  Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human colon. Positive cytoplasmic staining within epithelial cells of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human kidney medulla. Positive staining observes in a subset of in tubules and no staining within vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
p16INK4a / CDKN2A Antibody - Immunofluorescence of CDKN2A in Human Colon cells with CDKN2A antibody at 20 ug/ml.
p16INK4a / CDKN2A Antibody - Loading: 15 µg of lysates per lane. Antibodies: LS-B1347 (2 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
p16INK4a / CDKN2A Antibody - Loading: 10 µg of 293 WT cell lysates or KO cell lysates. Antibodies: LS-B1347 (2 µg/mL) and beta-actin (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human small intestine. Positive staining observed within the absorptive epithelium bordering the villi. Less staining observed within the lamina propria.   Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human spleen. Positive staining observed within a subset of lymphocytes.  Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human colon. Positive cytoplasmic staining within epithelial cells of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human kidney medulla. Positive staining observes in a subset of in tubules and no staining within vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
p16INK4a / CDKN2A Antibody - Immunofluorescence of CDKN2A in Human Colon cells with CDKN2A antibody at 20 ug/ml.
p16INK4a / CDKN2A Antibody - Loading: 15 µg of lysates per lane. Antibodies: LS-B1347 (2 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
p16INK4a / CDKN2A Antibody - Loading: 10 µg of 293 WT cell lysates or KO cell lysates. Antibodies: LS-B1347 (2 µg/mL) and beta-actin (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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IHC‑plus™ Polyclonal Rabbit anti‑Human p16INK4a / CDKN2A Antibody (IHC, IF, WB) LS‑B1347

IHC‑plus™ Polyclonal Rabbit anti‑Human p16INK4a / CDKN2A Antibody (IHC, IF, WB) LS‑B1347

Note: This antibody replaces LS-C34699, LS-C56942
Antibody:
p16INK4a / CDKN2A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$545
LS-B1347-0.05
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
p16INK4a / CDKN2A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CDKN2A antibody LS-B1347 is an unconjugated rabbit polyclonal antibody to CDKN2A (p16INK4a) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human p16INK4a / CDKN2A
Synonyms
INK4A | Multiple tumor suppressor 1 | MTS-1 | MTS1 | p16-INK4A | p16 INK4A | p16INK4A | CDKN2A | cyclin-dependent kinase inhibitor 2A
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Immunogen
An 18 amino acid synthetic peptide near the N-Terminus of human CDKN2A. The immunogen is located within the first 50 amino acids of CDKN2A.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (20 µg/ml)
  • Western blot (1 - 4 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
CDKN2A antibody can be used for detection of CDKN2A by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Western Blot: Predicted: 17 kDa Observed: 20 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human small intestine. Positive staining observed within the absorptive epithelium bordering the villi. Less staining observed within the lamina propria.   Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-p16INK4A antibody IHC of human small intestine. Positive staining observed within the absorptive epithelium bordering the villi. Less staining observed within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-p16INK4A antibody IHC of human small intestine. Positive staining observed within the absorptive epithelium bordering the villi. Less staining observed within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human spleen. Positive staining observed within a subset of lymphocytes.  Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-p16INK4A antibody IHC of human spleen. Positive staining observed within a subset of lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-p16INK4A antibody IHC of human spleen. Positive staining observed within a subset of lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human colon. Positive cytoplasmic staining within epithelial cells of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
Anti-p16INK4A antibody IHC of human colon. Positive cytoplasmic staining within epithelial cells of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
Anti-p16INK4A antibody IHC of human colon. Positive cytoplasmic staining within epithelial cells of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.

p16INK4a / CDKN2A Antibody - Anti-p16INK4A antibody IHC of human kidney medulla. Positive staining observes in a subset of in tubules and no staining within vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
Anti-p16INK4A antibody IHC of human kidney medulla. Positive staining observes in a subset of in tubules and no staining within vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
Anti-p16INK4A antibody IHC of human kidney medulla. Positive staining observes in a subset of in tubules and no staining within vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.

LSBio Ratings

IHC-plus™ p16INK4a / CDKN2A Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B1347 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)

Publications (3)

Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice. Zhang Y, Reddy A, Mohammed A, Lightfoot S, Dai W, Rao CV. Carcinogenesis. Mar; (IHC, IF; Mouse)

Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model. Sanghera S, Zhang Y, Biddick L, Reddy A, Lightfoot S, Dai W, Yamada HY. Molecular carcinogenesis. 2015 Mar; (IHC; Mouse)

Role of deltaNp63(pos)CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas. Yamano S, Gi M, Tago Y, Doi K, Okada S, Hirayama Y, Tachibana H, Ishii N, Fujioka M, Tatsumi K, Wanibuchi H. Cancer science. 2016 February;107:123-32. (IHC; Mouse)

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Requested From: United States
Date Requested: 5/23/2022