LSBio
IHC-plus™ Polyclonal Rabbit anti-Human NR4A1 / NUR77 Antibody (aa251-266, IHC, IF, WB)

Anti-NUR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human NR4A1 / NUR77 Antibody (aa251-266, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all NR4A1 / NUR77 antibodies →
$383.00each
Catalog Number: LS-B114-50

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Overview

Description
NUR77 antibody LS-B114 is an unconjugated rabbit polyclonal antibody to NUR77 (NR4A1) (aa251-266) from human. It is reactive with human, mouse and rat. Validated for ChrIP, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
ChIP, ICC, IF, IHC, IHC-P, IP, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
GFRP1 | Early response protein NAK1 | HMR | NGFIB | Orphan nuclear receptor TR3 | Orphan receptor tr3 | Steroid receptor TR3 | TR3 | NAK1 | NGFI-B | NP10 | NR4A1 | Receptor NGFIB | Testicular receptor 3 | TR3 orphan receptor | N10 | NAK-1 | NUR77 | Orphan nuclear receptor HMR | Tr3/NR4A1
Recommended Dilution: IHC-P
20 - 40 µg/ml
Recommended Dilution: IP
1:10 - 1:500
Recommended Dilution: Other
1:10 - 1:500
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P22736
SwissProt
P22736

Target

Target
NR4A1 / NUR77
Antigen Species
Human
Specificity
A synthetic peptide corresponding to aa 251-266 of human Nak1.
Epitope
A synthetic peptide corresponding to aa 251-266 of human Nak1.
Gene Name
NR4A1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from human NR4A1 / NUR77.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.05% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Nuclear receptor NR4A1 is a tumor suppressor down-regulated in triple-negative breast cancer. Wu H #, Bi J #, Peng Y #, Huo L #, Yu X, Yang Z, Zhou Y, Qin L, Xu Y, Liao L, Xie Y, Conneely OM, Jonkers J, Xu J,. Oncotarget. 2017;8:54364-54377.

PubMed: 28903348


Nur77 gene expression levels were involved in different ACTH-secretion autonomy between Cushing's disease and subclinical Cushing's disease. Tabuchi Y, Kitamura T, Fukuhara A, Mukai K, Onodera T, Miyata Y, Hamasaki T, Oshino S, Saitoh Y, Morii E, Otsuki M, Shimomura I. Endocrine Journal. 2016 June;63:545-554.

PubMed: 27025408

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