LSBio
IHC-plus™ Polyclonal Rabbit anti-Human NEDD8 Antibody (aa10-59, IHC, WB)

Anti-NEDD8 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHC-plus™ Polyclonal Rabbit anti-Human NEDD8 Antibody (aa10-59, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B6641-50

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Overview

Description
NEDD8 antibody LS-B6641 is an unconjugated rabbit polyclonal antibody to NEDD8 (aa10-59) from human. It is reactive with human, mouse and rat. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
NEDD8 | NEDD-8 | Neddylin | Ubiquitin-like protein Nedd8
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
Q15843
SwissProt
Q15843

Target

Target
NEDD8
Antigen Species
Human
Specificity
NEDD8 Antibody detects endogenous levels of total NEDD8 protein.
Epitope
NEDD8 Antibody detects endogenous levels of total NEDD8 protein.
Gene Name
NEDD8

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from human NEDD8. (AA Range: 8-59)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS, 150 mM NaCl
The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis. Xie P, Zhang M, He S, Lu K, Chen Y, Xing G, Lu Y, Liu P, Li Y, Wang S, Chai N, Wu J, Deng H, Wang HR, Cao Y, Zhao F, Cui Y, Wang J, He F, Zhang L. Nature communications. 5:3733. PubMed: 24821572

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