Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
HSPA5 / GRP78 / BiP Antibody (clone 3C5-1A4)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$444.00each
Catalog Number: LS-C774237-100
Catalog Number: LS-C774237
For bulk orders or custom formulations:
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- Description
- BiP antibody LS-C774237 is an unconjugated mouse monoclonal antibody to BiP (HSPA5 / GRP78) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78 | HSPA5
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P11021
- NCBI GenBank
- AAA52614.1, M19645
- SwissProt
- P11021
Target
- Target
- Human HSPA5 / GRP78 / BiP
- Antigen Species
- Hu
- Epitope
- Detects ~78 kDa.
- Gene Name
- HSPA5
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 3C5-1A4
- Isotype
- IgG1
- Light Chain
- κ
- Origin Species
- Hu
Immunogen
- Immunogen
- Full-length recombinant rat GRP78.
- Immunogen Type
- Recom
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS