Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Immunofluorescence of rabbit Anti-Histone H4 [Monomethyl Arg3] Antibody. Tissue: Nonmitotic, prophase, and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Monomethyl Arg3] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Monomethyl Arg3] is nuclear and chromosomal. Staining: Histone H4 [Monomethyl Arg3] is expressed in green, nuclei are counterstained with DAPI (blue).
HIST4H4 Antibody (Me-Arg3)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$594.00each
Catalog Number: LS-C745479-50
Catalog Number: LS-C745479
For bulk orders or custom formulations:
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- Description
- HIST4H4 antibody LS-C745479 is an unconjugated rabbit polyclonal antibody to HIST4H4 (Me-Arg3) from human. It is reactive with human, mouse and rat. Validated for ChrIP, IF, IHC and WB.
- Application
- ChIP, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HIST2H4 | Histone 4, H4 | Histone cluster 4, H4 | HIST4H4 | Histone H4
- Recommended Dilution: IF/ICC
- 1:50
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: IP
- 2 - 5 µg/10E6 cells
- Recommended Dilution: WB
- 1:1000
- NCBI GenBank
- NM_175054 | NP_778224.1
Target
- Target
- Human HIST4H4
- Antigen Species
- Human
- Epitope
- This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Histone H4 [Monomethyl Arg3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 3 of human Histone H4.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
- Concentration
- 0.64 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
