Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Immunofluorescence of rabbit Anti-Histone H3 [p Ser10] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Ser10] antibody at a 1:200 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Ser10] is nuclear and chromosomal. Staining: Histone H3 [p Ser10] is expressed in green, nuclei and actin are counterstained with Dapi (blue) and Phalloidin (red).
Polyclonal Rabbit anti-Human HIST3H3 Antibody (phospho-Ser10, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Chicken, Fruit Fly, Helminth / Nematode, Human, Mouse, Plant, Rat, Xenopus |
| Format: | Liquid |
$594.00each
Catalog Number: LS-C745457-50
Catalog Number: LS-C745457
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- HIST3H3 antibody LS-C745457 is an unconjugated rabbit polyclonal antibody to HIST3H3 (pSer10) from human. It is reactive with human, mouse, rat and other species. Validated for DB, IF, IHC and WB. Cited in 1 publication.
- Application
- DB, IF, IHC, WB
- Reactivity
- Ck, Dros, Helm, Hu, Ms, Plnt, Rt, Xen
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HIST3H3 | Histone H3.1t | H3t | Histone 3, H3 | Histone cluster 3, H3
- Recommended Dilution: IF/ICC
- 1:2000
- Recommended Dilution: IHC
- 1:2000
- Recommended Dilution: WB
- 1:500
- UniProt Number
- Q16695
- NCBI GenBank
- NM_003493, NP_003484.1
- SwissProt
- Q16695
Target
- Target
- Human HIST3H3
- Antigen Species
- Hu
- Epitope
- This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
- Gene Name
- H3-4
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Histone H3 [p Ser10] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 10 of human Histone H3.2.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
- Concentration
- 0.44 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Dpp-expressing and non-expressing cells: two different populations of growing cells in Drosophila. Carolina Arias, Gimena Fussero, Marcelo Zacharonok, Ana Macías. PloS one. 2015 March;10:e0121457. PubMed: 25798905 PMC: PMC4370563
