LSBio
His Tag Antibody (clone 33D10.D2.G8, HRP)

Western blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT. This image was taken for the unconjugated form of this product. Other forms have not been tested.

His Tag Antibody (clone 33D10.D2.G8, HRP)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Format:Lyophilized
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$564.00each
Catalog Number: LS-C154015-100

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Overview

Description
His Tag antibody LS-C154015 is an HRP-conjugated mouse monoclonal antibody to His Tag. Validated for ELISA and WB.
Application
ELISA, WB
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:10000
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
His Tag
Epitope
This antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
33D10.D2.G8
Isotype
IgG1
Light Chain
κ

Immunogen

Immunogen
This 6X his antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen Type
Fusion-P

Format

Conjugate/Label
HRP
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.